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| Registros recuperados: 44 | |
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| Berge, Jean-pascal. |
| La présence de substances immunologiquement apparentées aux O/CCK de vertébrés est détectée chez les crustacés décapodes (Favrel,1988; Turrigiano G.G,1989). Un protocole de purification est même établi pour Palaemon serratus et Nephrops norvegicus (Favrel,1988). Dans un premier temps, nous allons nous attacher à la purification des G/CCK immunologiquement apparentées aux hormones peptidiques de vertébrés (O/CCK"like") chez les deux espèces précitées. Dans un deuxième temps, à l'aide des fractions purifiées, le rôle biologique des G/CCK"Iike" et particulièrement leur action sur la synthèse protéique vont être étudiés. |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00136/24708/22762.pdf |
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| Fargeas, Valérie; Baalouch, Myriam; Metay, Estelle; Baffreau, Jerôme; Menard, Dominique; Gosselin, Pascal; Berge, Jean-pascal; Barthomeuf, Chantal; Lebreton, Jacques. |
| A simple and efficient methodology to introduce an 1,3-diketone motif from various aldehyde precursors in three steps with good overall yields is described using beta-ketosulphone 7 as masked equivalent of acetone. |
| Tipo: Text |
Palavras-chave: 1; 3 diketones Alkylation Sulfones Aldehydes. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-829.pdf |
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| Berge, Jean-pascal; Barnathan, Gilles. |
| Because of their characteristic living environments, marine organisms produce a variety of lipids. Fatty acids constitute the essential part of triglycerides and wax esters, which are the major components of fats and oils. Nevertheless, phospholipids and glycolipids have considerable importance and will be taken into account, especially the latter compounds that excite increasing interest regarding their promising biological activities. Thus, in addition to the major polyunsaturated fatty acids (PUFA) such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, a great number of various fatty acids occur in marine organisms, e.g. saturated, mono- and diunsaturated, branched, halogenated, hydroxylated, methoxylated, non-methylene-interrupted. Various... |
| Tipo: Text |
Palavras-chave: Nutrition; Biomarkers; Marine organisms; Fatty acids; Lipids. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-937.pdf |
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| Dubois, Nolwenn; Barthomeuf, Chantal; Berge, Jean-pascal. |
| It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation.... |
| Tipo: Text |
Palavras-chave: Sensitivity; Sterol esters; Fatty acid methyl esters; Gas chromatography; Mass spectrometry; Picolinyl esters. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2125.pdf |
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| Dumay, Justine; Donnay-moreno, Claire; Barnathan, G; Jaouen, P; Berge, Jean-pascal. |
| Enzymatic hydrolysis of sardine viscera by three broad spectrum proteases was investigated using the pHstat method (24 h, pH 8, 50 degrees C). After hydrolysis, three fractions (sludge, aqueous phase and oily phase) were collected. For each fraction, lipids and phospholipids were quantified and molecular weights of aqueous phase peptides were determined. Under these conditions, the degree of hydrolysis (DH) varied from 1.9% (Flavourzyme), 3.1% (Protamex) and to 3.3% (Alcalase). Dry matter distribution indicated that all hydrolysis performed increased solubilisation of proteins: protein recovery for each hydrolysis ranged from 57.4% to 61.2%. Furthermore, the total lipid content in the liquid parts (oily and aqueous phases) increased (at least 85% of lipids... |
| Tipo: Text |
Palavras-chave: Fish protein hydrolysate FPH; Sardine; By product; Lipid; Hydrolysis; Phospholipid. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2004.pdf |
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| Le Nechet, Sandrine; Dubois, Nolwenn; Gouygou, Jean-paul; Berge, Jean-pascal. |
| If rays are traditionally fished for their caudal fins, the rest of the body is wasted, except part of the skin that can be transformed into leather. Liver oil of the ray, Himantura bleekeri, was characterized in terms of lipid class composition and fatty acid profile. Liver oil content was high and represented 54% of the liver weight (w/w). Neutral lipids were predominant (92%), major components being triacylglycerols (63%). Other neutral lipids identified were hydrocarbons, sterol esters, mono- and di-acylglycerols, free fatty acids, sterols and glyceryl ethers. Polyunsaturated fatty acids of the n-3 series, namely eicosapentaenoic and docosahexaenoic acids, were high (4% and 16%, respectively). Cholesterol was the major component in the sterol fractions... |
| Tipo: Text |
Palavras-chave: Sterol; Squalene; DHA; EPA; PUFA; Lipids; Fatty acids; Ray liver oil. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3129.pdf |
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| Ruiz, Nicolas; Dubois, Nolwenn; Wielgosz Collin, Gaetane; Du Pont, Thibaut; Berge, Jean-pascal; Pouchus, Yves-francois; Barnathan, Gilles. |
| A marine-related Trichoderma longibrachiatum strain isolated from mussels in a farming shellfish area was investigated for total lipid (TL) production, total lipid fatty acids (TLFA), and phospholipid fatty acids (PLFA). Fungal biomass was produced from two different marine-like culture ways, on agar surface and submerged fermentations (ASF and SmF, respectively), allowing useful comparisons. ASF produced a rather higher biomass amount than SmF with similar TL content. All fatty acids (FA), identified by gas chromatography-mass spectrometry (GC-MS), were ranged from 14 to 30 carbon atoms. Similar FA compositions were found in TL and phospholipids (PL) from biomasses obtained by both fermentation systems, including oleic (up to 15.3% of total FA mixture for... |
| Tipo: Text |
Palavras-chave: Conjugated fatty acids; Fatty acids; Lipids; Submerged fermentation; Agar surface fermentation; Trichoderma longibrachiatum. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4199.pdf |
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| Thorkelsson, G; Sigurgisladottir, S; Geirsdottir, M; Jóhannsson, R; Guerard, Fabienne; Chabeaud, Aurelie; Bourseau, Patrick; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, M; Fouchereau-peron, Martine; Martinez Alvarez, Oscar; Le Gal, Yves; Ravallec-ple, Rozenn; Picot, Laurent; Berge, Jean-pascal; Delannoy, Charles; Jakobsen, Greta; Johansson, I; Batista, Ireneu. |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00056/16747/14184.pdf |
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| Sylla, K. S. B.; Musabyemariya, B.; Berge, Jean-pascal; Seydi, Mg. |
| The by-products (viscera, heads, backbone and skins) of tongue sole coming from a Senegal frozen fisherie plant, were hydrolized with the Protamex® enzyme.Degrees of hydrolysis of 30 and 19% were obtained respectively with 40°C and 50°C.The results also showed that the proteins contained in these by-products are completely solubilized by the action of this protease at the end of 180 minutes of hydrolysis. The biochemical characterization showed that these hydrolysates are rich in proteins (61%) and minerals (10%). However, the hydrolysates contain peptides of lower molecular sizes 1760Da.Lastly, these hydrolysates have high nutritional value in order to set up table fowls because they have in big quantity 9 essential amino acids for the poultry feed. |
| Tipo: Text |
Palavras-chave: Co-produits; Hydrolyse enzymatique; Protéines; By-products; Enzymatic hydrolysis; Proteins. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00015/12621/9506.pdf |
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| Perez-galvez, Raul; Berge, Jean-pascal. |
| Introduction : A direct consequence of traditional fishing practices is the considerable quantities of wastes and byproducts that are generated. They represent an economical and environmental problem since not all obtained from the sea is adequately used but thrown back into the sea as a waste. With the aim of classifying and quantifying the by-products, three main groups are considered: discards, wastage on board and by-products and wastage on shore. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7409.PDF |
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| Bourseau, Patrick; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, Maryse; Masse, Anthony; Guerard, Fabienne; Chabeaud, Aurélie; Fouchereau-peron, Martine; Le Gal, Yves; Ravallec-ple, Rosenn; Berge, Jean-pascal; Picot, Laurent; Piot, Jean -marie; Batista, Ireneu; Thorkelsson, Gudjon; Delannoy, Charles; Jakobsen, Greta; Johansson, Inger. |
| The production by enzymatic treatment of fish protein hydrolysates (FPH) is a promising route to add value to fisheries proteinic co-products (fish frames, heads etc.). Indeed, FPH possess good nutritional properties and biological activities for food and feed uses. Pressure-driven membrane separations such as ultrafiltration (UF) and nanofiltration (NF) can be used after the hydrolysis to, increase the specific activities of the FPH. This paper discusses the impact of a two-step UF/NF process producing four different fractions on two industrial FPH with different hydrolysis degrees. Fractionation is carried out in "realistic" conditions for an industrial process, on highly concentrated FPH solutions (about 100 g of dry matter/L) at a high volume reduction... |
| Tipo: Text |
Palavras-chave: Peptidic profile; Fractionation process; Membrane separation; Nanofiltration; Ultrafiltration; FPH; Fish protein hydrolysate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6653.pdf |
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| Bourseau, Patrick; Chabeaud, Aurélie; Vandanjon, Laurent; Masse, Anthony; Jaouen, Pascal; Fleurence, Joël; Berge, Jean-pascal. |
| This review reports some interesting works on the recovery and fractionation by membrane techniques of marine compounds resulting from hydrolysis of fish by-products such as proteins or peptides, lipids or fatty acids, and carbohydrates. Ultrafiltration (UF) appears as the most widely used technique for such applications. It must be noted that only a few works deal with the optimization or the enhancement of processes for the recovery of added value molecules. Indeed, most of works use UF as a convenient and simple tool for solute fractionation, for this reason the influence of operating parameters on membrane separation efficiency is unfortunately seldom considered. When attention is paid in a study to the process development, it is specified in this... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7408.pdf |
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| Kechaou, Emna; Durnay, Justine; Donnay-moreno, Claire; Jaouen, Pascal; Gouygou, Jean-paul; Berge, Jean-pascal; Ben Arnar, Raja. |
| Total lipid and phospholipid recovery as well as amino acid quality and composition from cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) were compared. Enzymatic hydrolyses were performed using the three proteases Protamex, Alcalase, and Flavourzyme by the pH-stat method (24 h, pH 8, 50 degrees C). Three fractions were generated: an insoluble sludge, a soluble aqueous phase, and an oily phase. For each fraction, lipids, phospholipids, and proteins were quantified. Quantitative and qualitative analyses of the raw material and hydrolysates were performed. The degree of hydrolysis (DH) for cuttlefish viscera was 3.2% using Protamex, 6.8% using Flavourzyme, and 7% using Alcalase. DH for sardine viscera was 1.9% (using Flavourzyme), 3.1% (using... |
| Tipo: Text |
Palavras-chave: Sardine; Viscera; Proteolysis; Marine lipids; Cuttlefish; By products. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6366.pdf |
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| Dubois, Nolwenn; Barnathan, Gilles; Gouygou, Jean-paul; Berge, Jean-pascal. |
| The gas chromatographic properties of four derivatives of fatty acids (FA), namely fatty acid methyl esters, picolinyl esters, N-acyl pyrrolidides and 4,4-dimethyloxazoline derivatives, which contain various structural features (double bonds, branching, hydroxyl group) in their acyl chains have been compared on a low-polarity capillary column with a mass spectrometer as detector. Temperature programming rates yielding the highest resolution were optimized for each derivative by means of computer-assisted column temperature optimization software. Indeed, the Drylab software represents a valuable assistance for estimating the optimum analysis conditions. Time and efforts required for such method development can greatly be reduced. Different parameters... |
| Tipo: Text |
Palavras-chave: Picolinyl esters N Acyl pyrrolidides Methyl esters GC MS Fatty acid derivatives 4; 4 Dimethyloxazoline. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6846.pdf |
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| Perez-galvez, Raul; Chopin, Christine; Mastail, Max; Ragon, Jean-yves; Guadix, Antonio; Berge, Jean-pascal. |
| Fresh sardine (employed as discard model species) was pressed with a hydraulic press in order to obtain a partially dewatered cake (whose volume represents between 55% and 60% of the initial Volume of raw material) and a 3-phase press liquor comprising an aqueous fraction, an oil fraction and a variable amount of suspended solids. Pressing conditions, such as final pressure to which the sardine was subjected, compression speed, number of pressing stages and time of relaxation between two consecutive pressing stages, were optimised by means of a statistically designed experiment. The goal of the optimisation problem was to achieve maximal yield of press liquor with a minimum load of suspended solids. The Suspended solid content in the press liquor Must be... |
| Tipo: Text |
Palavras-chave: Multiobjective optimisation; Response surface methodology; Hydraulic pressing; Fish discards; Sardine. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7415.pdf |
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| Registros recuperados: 44 | |
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